BIOL 434 Basic Principles & Methods in Molecular Genetics

Prerequisite: BIOL 316 or permission of the instructor. This integrated lab-lecture course provides basic concepts and hands-on experience with common molecular genetics and recombinant DNA methods. Topics include techniques for the isolation of DNA and RNA, gene cloning employing plasmid vectors, DNA sequencing, polymerase chain reaction (PCR) technology, expression of fusion proteins in E. coli, and web-based analysis of sequence data.