BMS 535 Advanced Topics in Recombinant DNA Technology: Gene Transfer, Expression, and Detection

Prerequisite: BMS 534 or permission of the instructor. Lab fee. A study of the theory and techniques for the introduction, expression and detection of macromolecules in eukaryotic cells. The topics to be covered include the introduction of recombinant genes in eukaryotic hosts. Methodologies to produce transgenic animals will be discussed. Analysis of eukaryotic gene expression, detection of activity by western analysis and cell-based assays